Squished lanes in SDS Page
Posted 14 September 2010 - 03:00 AM
got the following prob:
Run 9 samples (all processed the same way) in SDS Page under following conditions:
Nupage Bis-Tris 4-12% precast gels and MOPS buffer from Invitrogen
The samples are cells washed once in PBS, lysed in RIPA + PIs, added Laemmli (reducing)
cooked 5min 99deg, chilled on ice and loaded onto gel
And what I get is 7 of 9 samples running very strangely on the gel (have a look at attached pic).
First thought samples were degraded but....
Only thing I didn't do is spin after cooking the samples
Or too high/low salt concentration?
Posted 17 September 2010 - 08:35 AM
you can reduce salt in a small aliquot for sds-page by drop dialysis.
Edited by mdfenko, 17 September 2010 - 08:37 AM.
genius does what it must
i do what i get paid to do
Posted 17 September 2010 - 02:43 PM
I always had an alternate hypothesis....
Posted 18 September 2010 - 08:10 PM