Have been going through a few papers (1988-1993). I would like to repeat a few experiments from there. However, all papers mention that to sequence the genes of interest, they amplified the DNA using specific primers and cloned the PCR product into some phage DNA, before it was sequenced.
Is it necessary to clone the DNA before sequencing, even today??? Would they then do it because that sequencing techniques were not well developed???
Edited by gt_ameya, 13 September 2010 - 09:51 PM.