I would like to design a negative control siRNA. I've been told to basically just scramble the nucleotide sequence of my gene-specific siRNA.
The problem is, the genome for my model organism has not been sequenced. Therefore, I cannot search to see whether my scrambled control lacks homology with another gene. How can I prevent accidental knockdown of another gene with my control?
Thanks in advance for any suggestions!
How to Design a Negative Control siRNA
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