Protin helicase (urgent)
Posted 13 September 2010 - 02:28 PM
Posted 13 September 2010 - 04:21 PM
Posted 13 September 2010 - 05:55 PM
it is a plasmid of a protein that possess helicase activity.
You have a plasmid that encodes helicase? Or do you you have some helicase that has been stored for 7 years? For the former, it should be fine, unless the plasmid has degraded as the plasmid will generate fresh helicase on transfection. For the latter, you should check the activity before using.
Posted 13 September 2010 - 07:47 PM
Is the plasmid stored as DNA or is it frozen in a host strain (of E. coli, perhaps)?
If it has been stored as DNA, transform a compatible bacterial strain, recover fresh plasmid DNA, and have the insert sequenced to insure integrity of the DNA.