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Guanadine HCl for plasmid miniprep


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#1 tarquin

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Posted 13 September 2010 - 09:29 AM

Hello

We have to buy GuHCl to use for high throughput plasmid minipreps before sequencing. Many commercial kits use the same chemical as a bind solution for binding DNA to the filter plate.

The problem is cost. 100g of molecular grade(99%) GuHCl is R$200, but this is enough for only 4 plates. Whereas 1KG of "technical grade"(98%) GuHCl is the same price yet enough for ~48 plates.

Do you think there would be a problem to use technical grade? Its for a 6M solution to add to the cell lysates following Solutions I,II, and III in standard miniprep.

Many thanks

#2 ranvi

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Posted 13 September 2010 - 05:13 PM

y cant you buy mini prep kit which is cheaper $107 for 50 minipreps qiagen

#3 tarquin

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Posted 14 September 2010 - 03:23 AM

y cant you buy mini prep kit which is cheaper $107 for 50 minipreps qiagen


We have to do high throughput 96 well plates. Although the Qiagen plate miniprep is the cheapest here in Brazil, it's still bloody expensive. It's cheaper for us to buy Millipore plates and make our own solutions.

#4 perneseblue

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Posted 17 September 2010 - 04:40 PM

I personally feel it will be okay.

But I guess the smart thing is to buy or ask the company for a small sample of the 98% Guanidine HCl and see if the DNA extracted using this will sequence.

I use 98% Guanidine thiocyanate to extract my DNA sample. However I later phenol-chloroform purify the DNA.
May your PCR products be long, your protocols short and your boss on holiday




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