band diffusion in sds
Posted 13 September 2010 - 06:33 AM
Posted 13 September 2010 - 11:27 AM
Posted 14 September 2010 - 09:27 PM
Posted 16 September 2010 - 03:38 AM
Are you running the same amount of protein in each well? When I run a sample with lots of protein, it tends to spread out and squeeze the lanes around it. Also, what size is your purified protein? My bands tend to get broader in lower percentage or at the bottom of a gradient gel. If you are running a lot of protein, it may help to load a lot of sample buffer in the surrounding lanes.
size of my protein is 55 kda, 12% gel.
Posted 16 September 2010 - 03:40 AM
how does salt affect the migration.MY ion exchange has .5M nacl and NINTA elute ha 300 mM imidazole. When they move on gel they make a curve.
Hola, It could be due at high salt concentration. For instance, when you run the elution fractions of an ion exchanger, as much higher salt concentration much wide bands . Good luck
Posted 16 September 2010 - 10:32 PM