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Is Histone H1 suitable to check the purity of cytoplasm extracts?


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#1 nocas

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Posted 13 September 2010 - 02:48 AM

Hello everyone,

I've read in this forum that some people use histone as a marker to check the purity of cytoplasmic extracts but I only saw references to histone H3. The problem is that the antibody that I have in my lab is for histone H1 and I read that this histone is present in the cytoplasm. So I would like to know if anyone has used anti-H1 to check for puryity of cytoplasmic extracts with success. My extracts are from a monocytic cell line (THP-1).

Thanks in advance

#2 bob1

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Posted 13 September 2010 - 04:15 PM

If it is found in the cytoplasm as well as the nucleus, it is not suitable for checking the fractionation.




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