Determining whether the quality of an antibody and if it is species specific
Posted 12 September 2010 - 08:45 PM
Thanks in advance from a very confused freshman!
Posted 13 September 2010 - 12:30 PM
Posted 13 September 2010 - 04:13 PM
If it is protein, you should determine if the antibody is binding to the protein specifically by performing competition experiments where you incubate the antibody with a peptide corresponding to the epitope of the antibody and then see if it will bind to the target protein. You should titrate the amount of peptide used to show increasing competition. This is best done on western blots.