Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

pre-miRNA problems

  • Please log in to reply
No replies to this topic

#1 miRNAnewbie



  • Members
  • Pip
  • 2 posts

Posted 12 September 2010 - 02:20 PM

Hi guys, I am trying to use pre-miRs from ambion to knockdown my target (which was confirmed by luciferase reporter assay), but by looking at the endogenous protein, I actually get opposite results...instead of my target decreasing, it is actually increasing.

any suggestions as to why this might be happening, I tried both 10 and 20nM for the pre-miRs


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.