Can any body guide me how I can make a pH gradient 3-8 by simple buffers (not empholytes) chromatfocussing on anoin exchange column.
waiting to Thankyou,
1 reply to this topic
Posted 13 June 2002 - 08:28 AM
Angelika Gorg (umlaut over the o), the princess of proteomics and 2-D gel electrophoresis has considerable information on creating IEF 1-D gels and how to make a 3-10 strip. Check her out online. CAs might be an unavoidable necessity.