QUESTION-CAPLE OF siRNAs sequences
Posted 12 September 2010 - 06:17 AM
I used 2 different siRNAs sequences to knockdown the same mRNA.
they both gave similar level of knockdown ,but the downstream effects (other proteins that were influenced)were not the same.
how is it possible? i compared them to the database-and they not supposed to knockdown any other gene. what can i do except try another sequence?
help me ...
Posted 18 September 2010 - 09:50 PM
Posted 24 September 2010 - 04:07 PM