Immunofluorescence: polyclonal vs monoclonal antibodies and negative controls
Posted 11 September 2010 - 08:52 AM
I have a few questions about immunofluorescence.
Is it better to use a monoclonal or a polyclonal primary antibody?
Also, how do I verify for autofluorescence and background fluorescence?
Thank you very much.
Posted 11 September 2010 - 02:39 PM
Posted 12 September 2010 - 08:25 AM
Posted 23 September 2010 - 01:37 PM
IgG is a good control but the blocked antibody seems to be the best one because with IgG you do not really know where they come from.. I tell that because I have problem with using IgG as a control for immunofluorescence. Now I am trying to block my first antibody.
Thank you very for your answer. I makes a lot of sense. However, what about replacing the primary antibody with the IgG from the species in which it was raised. Would it serve as a good control for the specificity of my antibody?
Posted 24 September 2010 - 10:15 AM