Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

ChIP/qPCR: IgG control, dilution of samples and enrichement

  • Please log in to reply
No replies to this topic

#1 bioche82



  • Active Members
  • Pip
  • 5 posts

Posted 11 September 2010 - 08:31 AM

Hello guys,

I have a few questions about ChIPs and quantitative real-time PCR.
When performing RT-PCR on ChIP samples, why do I have to dilute my samples 1/5? Is it because elements in the samples might affect the amplification and why would that be?

My other question is why exactly is the IgG control for and what could explain that I have a significant amplification for my IgG samples?

Finally, what could explain that I obtain no amplification at all with my qPCRs when I expected a binding of my transcription factor to my target DNA sequence?

Thank you very much in advance.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.