MDA-MB-231 cells question
Posted 10 September 2010 - 03:05 PM
Just ordered MDA-MB-231 cells from ATCC and L-15 media for it. Their web site says that cells should be in 100% air, 37C conditions. Later, I found online that some people grow these cells in regular 5% CO2, 37C incubators using DMEM media with 10% FBS. So, which is it???
Thanks in advance.
Posted 10 September 2010 - 04:15 PM
Posted 11 September 2010 - 12:53 PM
Posted 13 September 2010 - 09:03 AM
I just checked ATCC and your correct 100% atmosphere w/o CO2. I've grown these cells before, but it has always been under 95%/5%CO2. Like Bob said, I would grow them under the conditions that ATCC recommends, but in addition you may want to make freezebacks in this initial state i.e. the first couple of passages in case something goes wrong as you accommodate the cells to different conditions. In the past, people have acclimated the cells to grow in other mediums including IMEM, DMEM and RPMI in addition to the starting medium you mentioned. Good Luck!