Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Embedding of early stage mouse embryos in OCT


  • Please log in to reply
No replies to this topic

#1 mamoo

mamoo

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 10 September 2010 - 01:37 AM

Hi Everyone,

I'm embedding E7.5- E9.5 mouse embryos into OCT for snap freezing and cryosectioning. I'm able to embed them into OCT without much difficulties but I'm unable to locate the embryo during my sectioning, even though I try to place them near the top of the block. The embryos are fixed in 2% PFA/30% sucrose for 2 hours and washed with 30% sucrose overnight.

All help will be greatly appreciated. Thanks!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.