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Primary cerebellar culture

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#1 katenkak



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Posted 09 September 2010 - 11:29 PM

Hi all!
Does anyone has an experience with long-term culture of mouse cerebellar neurons? I would like to make one, but I can't find protocol where KCl is NOT used. Is it really hopeless to get the culture without KCl? I want to study synaptic proteins expression and don't know how KCl might affect it. Please share your experience. Thanks!

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