Edited by patchbear, 09 September 2010 - 06:37 AM.
What’s wrong with my NTC
Posted 09 September 2010 - 06:36 AM
Posted 09 September 2010 - 07:18 AM
1. Buy new primers. This background band could well be your primers coming together in some weird structure that happens to run at this size
2. Design a new assay. Having a band at 150 bp is very close to where all the issue arise. Design an assay that gives you a larger band, at least 300 bp.
My two cents
- patchbear likes this
Posted 09 September 2010 - 04:19 PM
but what's the size of the pds?there are two bands in my NTC,one is primer dimer, the other is weird structure ?