why do we use only SDS in PAGE?? why cant it be some other detergent like butanyl sulfate??
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#2
mdfenko
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Posted 09 September 2010 - 06:44 AM
sds was found to be highly efficient in solubilizing and dissociating protein complexes. that and its ability to part a (roughly) equivalent charge density to the proteins makes it useful for separations based on size.
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#3
anuj
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Posted 09 September 2010 - 10:29 PM
mdfenko, on 09 September 2010 - 06:44 AM, said:
sds was found to be highly efficient in solubilizing and dissociating protein complexes. that and its ability to part a (roughly) equivalent charge density to the proteins makes it useful for separations based on size.
thnx for ur reply...bt m sory to say i dont find it to be satisfactory ans..
#4
mdfenko
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Posted 10 September 2010 - 07:12 AM
the long hydrophobic tail wraps around the hydrophobic parts of the protein.
for more, see these references (i don't have access):
shapiro et al, bbrc (1967)
sds page, encyclopedia of immunology
one dimensional sds page, encyclopedia of separation science
for more, see these references (i don't have access):
shapiro et al, bbrc (1967)
sds page, encyclopedia of immunology
one dimensional sds page, encyclopedia of separation science
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
