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colony PCR for subcloning gene
1 reply to this topic
Posted 08 September 2010 - 10:11 PM
I want to subclone a gene from the E coli genomic DNA into an expression vector. Can I simply do a colony PCR with primers containing the restriction sites to amplify the gene or do I have to purify the genomic DNA first? Thanks!
Posted 09 September 2010 - 02:46 AM
If your primers produce a single well-isolated band of sufficient quantity when used in colony PCR, you can recover this band from a gel and clone it.