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colony PCR for subcloning gene


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#1 donny

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Posted 08 September 2010 - 10:11 PM

I want to subclone a gene from the E coli genomic DNA into an expression vector. Can I simply do a colony PCR with primers containing the restriction sites to amplify the gene or do I have to purify the genomic DNA first? Thanks!

#2 HomeBrew

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Posted 09 September 2010 - 02:46 AM

If your primers produce a single well-isolated band of sufficient quantity when used in colony PCR, you can recover this band from a gel and clone it.




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