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Mammalian secreted protein disappears after EndoF, TeV treatment

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#1 ranvi



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Posted 08 September 2010 - 01:40 PM


I am trying to purify the mammalian secreted protein, I can see fair amount of protein after ni purification but it undergoes proteolysis. I treated with TEV and ENDO F to cleave off the tags and after that I run on commassie and Westernblot and I dont see my protein any more!! all I see is only TEV what could be happening?

Thanks for any help

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