thawed cells wont adhere
Posted 08 September 2010 - 10:53 AM
Posted 08 September 2010 - 06:02 PM
1. are you using tissue culture treated flasks?
2. are you SURE they are viable? How have you decided/checked this?
3. are you using the right media formulation for each cell type?
Now to look at each cell line:
if you look at the ATCC info for your PC3 line here (http://www.atcc.org/...ate=cellBiology) you will see that these cells can be adapted for suspension culture- is there anyone in your lab you can check with to see if this has been done for your batch? (also worth checking how this is done, perhaps your culture conditions are contributing?)
again from ATCC (http://www.atcc.org/...ate=cellBiology). These cells don't adhere very well and so you shouldn't do anything to them 48hours after thawing to allow them to settle and start to adhere
Hope this helps
Posted 09 September 2010 - 02:14 AM
For cells that do not adhere to normal TC plastic, you can use:
Primaria (special TC plastic from BD).