cloning of an operon
Posted 08 September 2010 - 02:08 AM
Im trying to clone and express the riboflavin biosynthesis operon in E. coli. There are 4 overlapping genes and each contains it start and stop codon, and the total length of this is 8 kb. Is it possible to amplify 8 kb using Pfu?. Also is is possible to clone the operon in pET30 plasmid and express in E. coliBL21?
Posted 08 September 2010 - 03:04 AM
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
Posted 08 September 2010 - 03:07 AM