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Cloning to make expression vector


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#1 mcb56

mcb56

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Posted 07 September 2010 - 07:46 AM

I would like to clone in a PCR amplified insert so that it will be driven by an actin promoter in a vector I am using. How close does the insert have to be to the actin promoter to make sure that the expression of my insert is driven by it? The place I would like to insert it is about 150 bp upstream of the end of the promoter region.

Thank You




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