Posted 07 September 2010 - 07:24 AM
I have two general questions:
- Does anybody know why is sometimes so difficult to reamplify a PCR product with the same primers, and sometimes it is easy?
- Why is a nested PCR usually better in order to reamplify? What happens at the end of the PCR product that makes it easier for a nested primer to anneal?
Thanks in advance!
Posted 07 September 2010 - 07:51 AM
My two cents
Posted 07 September 2010 - 08:01 AM
Thanks for your quick reply, but I forgot to say that I usually clean my PCR product with a Qiagen kit (MinElute PCR purification kit), so I guess that in my case, this is not the problem.