Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Staining and destaining PAGE gels


  • Please log in to reply
4 replies to this topic

#1 kadamb

kadamb

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 08 July 2002 - 09:48 PM

Hi! i am running PAGE gels  almost everyday. I am using standard staining method which contains methanol, As methanol is very toxic i am looking for any other alternative method which does not involves methanol. Any suggestions.

#2 sbidw

sbidw

    member

  • Active Members
  • Pip
  • 16 posts
0
Neutral

Posted 12 July 2002 - 02:43 AM

You can use ethanol instead of methanol, but they take an age to  stain and destain - not recommended unless youve got plenty of time of your hands. You can buy premade methanol free satin from invitrogen and biorad but it ain't cheap.  I would just stick with the methanol and  make an effort to be carefull.

#3 Megan

Megan

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 28 July 2002 - 01:06 PM

Hi kadamb :-)
in our lab we are using the "Gel Code Blue Stain Reagent" from Pierce. It is easy to use and less toxic, the destaining is done with water.
for more info:
http://www.piercenet...am=02051015<p>I hope that helps you,
greetz
Megan

#4 takattacker

takattacker

    member

  • Active Members
  • Pip
  • 6 posts
1
Neutral

Posted 24 September 2002 - 01:51 PM

How dangerous is methanol anyway? Will I die if I drank a drop of it?

#5 jadefalcon

jadefalcon

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 223 posts
1
Neutral

Posted 13 November 2002 - 08:49 AM

Methanol has an LDLo human of 143 mg/kg.

LDLo is an abbreviation for Lethal Dose Low, the minimum amount of a chemical which tests have shown will be lethal to a specified type of animal. This is normally quoted in mg kg-1 body weight.

For comparison, KCN has a LDLo human of 2,86 mg/kg.

We use Ethanol instead of Methanol in all (Coomassie-) solutions and we put the gel in the staining solution, boil it twice for 20'' in the microwave for staining. For destaining, we let it cool down a bit in destaining solution (about 5') then cange the solution, and boil it twice for 20'' again, then change buffer again and destain at RT for about 1h.

Mike

Never give up, never surrender!
--- He who finds typos may keep them! ---




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.