Staining and destaining PAGE gels
Posted 08 July 2002 - 09:48 PM
Posted 12 July 2002 - 02:43 AM
Posted 24 September 2002 - 01:51 PM
Posted 13 November 2002 - 08:49 AM
LDLo is an abbreviation for Lethal Dose Low, the minimum amount of a chemical which tests have shown will be lethal to a specified type of animal. This is normally quoted in mg kg-1 body weight.
For comparison, KCN has a LDLo human of 2,86 mg/kg.
We use Ethanol instead of Methanol in all (Coomassie-) solutions and we put the gel in the staining solution, boil it twice for 20'' in the microwave for staining. For destaining, we let it cool down a bit in destaining solution (about 5') then cange the solution, and boil it twice for 20'' again, then change buffer again and destain at RT for about 1h.
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