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eliminating albumin from samples for western blotting


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#1 Paola PC

Paola PC

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Posted 01 August 2002 - 09:04 AM

I am starting to study a protein which is secreted from cultured mammalian cell
                      lines. I need to concentrate the conditioned medium to do some Westerns. The
                      problem is that this way I have so much albumin from serum in my samples which
                      makes a "blob" in the gel... How could I get rid of it? Is there any affinity
                      chromatography I can use to absorb albumin?
                      my protein is about 30 KDa and pI is 6.66; albumin is 68KDa and pI is 5.8
                      Thanks




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