Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

HaCaT cells


  • Please log in to reply
2 replies to this topic

#1 sspharm

sspharm

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 06 September 2010 - 06:41 AM

Hi

Any ideas why do HaCaT cels respond weakly to drugs? I incubate cells for 24 hours with my drug and when response is measured against control cells, response is in the rnge of 12-20 % only. any ideas?

Thanks in advance

#2 FlicFlak

FlicFlak

    member

  • Active Members
  • Pip
  • 6 posts
1
Neutral

Posted 06 September 2010 - 06:50 AM

Have you checked the soluability of your drug? Depending on the hydro/lipophilicity of the cell line and drug, uptake may not happen over a short time.

#3 sspharm

sspharm

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 06 September 2010 - 07:31 AM

Thanks for your reply. I've tried incubating the drug with cells for 1 , 2 hours only. Nearly same cell response. May I'd 've tried longer incubation times.



Have you checked the soluability of your drug? Depending on the hydro/lipophilicity of the cell line and drug, uptake may not happen over a short time.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.