Posted 06 September 2010 - 06:41 AM
Any ideas why do HaCaT cels respond weakly to drugs? I incubate cells for 24 hours with my drug and when response is measured against control cells, response is in the rnge of 12-20 % only. any ideas?
Thanks in advance
Posted 06 September 2010 - 06:50 AM
Posted 06 September 2010 - 07:31 AM
Have you checked the soluability of your drug? Depending on the hydro/lipophilicity of the cell line and drug, uptake may not happen over a short time.