3 replies to this topic

[brayuk]

Hi,

I was wondering if anyone has come across the following problem.  I want to make media in a 0.20µm filter unit (vacuum) and then freeze the receiver at -80C but I'm really struggling to find something suitable.  

I've found bottles with a couple of companies which will store media at this temperature, but none which connect to a filter.  Size isn't too much of an issue but anything in the 250-500ml range would be ideal.  Does anyone know of anything which fits this discription?

Thanks.

[leelee]

Can't you just filter into a sterile container and then transfer into something (sterile) that you can freeze?

[sgt4boston]

I would transfer to sterile container.  Those polystyrene containers will probably crack at -80C. Polyprob bottles would be best, do not fill to the top leave some headspace for expansion.

[brayuk]

Thanks for the responses.  The only reason I was looking for a receiver which doubles up as a freezer storage vessel is that my lab kis upping its waste minimising policy and I was trying to streamline.  If there's nothing suitable on the market, it can't be helped!
Thanks again