I'm confused :S
I standardized everything for my real time PCR experiment by doing regular PCRs, and got very nice amplicons both for the standard curves and cDNA. However, when I run my qRT-PCR plate with ssoFast Evagreen, I got no amplification signal for the whole plate!! No even the standard curve :S To rule out a problem with the detector I ran the products on an agarose gel with ethidium bromide and saw no amplification product, just the smears for the cDNA. Any suggestions would be truly appreciated!!
help, no amplification with SsoFast Evagreen
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