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restriction enzyme digestion

Started by guilda, Sep 05 2010 10:06 AM

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#1 guilda

guilda

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Posted 05 September 2010 - 10:06 AM

Dear All
Hi

I had a RFLP-PCR, I want to differenciate mutant allel from wild allel. After digestion with enzyme for wild or mutation, I found wild band is sharper than the mutant band. why? Please help me.

thank you
Guilda
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