2 replies to this topic

[autoclave]

Hi Everyone,

Have got a question which I think might be quite obvious, but I'm not 100% sure as I am a newcomer to all this. Here it goes -->

I have downloaded a set of nucleotide sequences for a specific gene I'm interested in (FASTA format). Some of the sequences say  "+ strand" and some say "- strand". It would seem obvious to me that before aligning the sequences to design my probe/primers, I would transform the + strand to its complement. I would just like to know if this is correct.

Thanks a lot!

a

[Curtis]

just use NCBI's primer design tool on its website and you don't need to confuse yourself. the forward primer binds to - strand, and reverse primer to + strand.

[susanna]

yes, u can use primer3-blast on NCBI

Curtis, on 06 September 2010 - 12:15 AM, said:

just use NCBI's primer design tool on its website and you don't need to confuse yourself. the forward primer binds to - strand, and reverse primer to + strand.