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Primer Design


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#1 autoclave

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Posted 05 September 2010 - 05:03 AM

Hi Everyone,

Have got a question which I think might be quite obvious, but I'm not 100% sure as I am a newcomer to all this. Here it goes -->

I have downloaded a set of nucleotide sequences for a specific gene I'm interested in (FASTA format). Some of the sequences say "+ strand" and some say "- strand". It would seem obvious to me that before aligning the sequences to design my probe/primers, I would transform the + strand to its complement. I would just like to know if this is correct.

Thanks a lot!

a

#2 Curtis

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Posted 06 September 2010 - 12:15 AM

just use NCBI's primer design tool on its website and you don't need to confuse yourself. the forward primer binds to - strand, and reverse primer to + strand.

#3 susanna

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Posted 17 September 2010 - 12:14 AM

yes, u can use primer3-blast on NCBI

just use NCBI's primer design tool on its website and you don't need to confuse yourself. the forward primer binds to - strand, and reverse primer to + strand.






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