1 reply to this topic

[jehane]

HELLO all

i want a simple method for quantifying total lipids in tissue sample. (Muscle and Liver)

Best regards.

[wilson_trace]

jehane, on 04 September 2010 - 05:57 AM, said:

HELLO all

i want a simple method for quantifying total lipids in tissue sample. (Muscle and Liver)

Best regards.

Hi,
Most of the analytical techniques can be used to analyze lipids including fatty acids, phospholipids,  sphingolipids,  triglycerides and steroids. Some of the faster technologies used today include GC-MS, LC-MS, nano ESI-FTMS. FT-MS or Orbitrap technologies allows quick profiling (1 minute acquisition time) of lipids from crude extracts of plasma or tissue samples (plants, humans, bacteria, algea).
You can access the paper from: http://findarticles....ag=content;col1

With advent of these technologies, many tools have also been developed to analyze the lipidome. SimLipid is one such software:  http://www.premierbi...ipid/index.html

Wilson  

Edited by wilson_trace, 15 February 2011 - 09:16 PM.