bacteria inhibiting Staphyllococcus aureus
Posted 04 September 2010 - 09:04 AM
Posted 06 September 2010 - 09:44 PM
Posted 07 September 2010 - 08:43 PM
i checked my supernantant with ammonium sulphate. 40, 60 and 80 percent. but did not get any precipitate at 40 and 60. at 80 there were precipitates but are very difficut to centrifuge. so i m confused hot to get them extracted.
rest when dissolved ppt in buffer they gave activity against S.aureus
problem for now is to extract all ppt then will go for SDS
rest u suggest..
Posted 07 September 2010 - 10:19 PM
Posted 08 September 2010 - 07:50 PM
thanks for suggestions. I have plans to dialize it. I have run simple native gel but never SDS so will seek help again.
I will do it after few days as I have to attend a workshop next week. so will come to topic after few days.