2 replies to this topic

[isabellaweng]

I have been culturing cells more than one year, cultured around 20 kinds of cells, but recently, I found my lncap cells and MCF-7 cells are ethanol-sensitive, when treated with 0.1% ethanol, they all died, but 0.01%ethanol group is OK, 0.05% group, half of the cells died. I repeated 2-3 times, the same thing happened. but, years ago, there was another person doing experiments with lncap cells in our lab, she used 0.2% ethanol, the cells were still OK(both my cells and her cells are from the same original vial ordered from ATCC), I don't know what happened to these cells? Does anyone have this kind of experience?
PS: At the same time, I am using LAPC-4, HAEC cells doing experients, they are OK when using 0.1% ethanol.

[bokashi farmer]

I dont know what is Incap cells, do you mind explaining?

I have similar problem with MCF-7. I start to notice that MCF-7 is might be sensitive to ethanol. I made it custom to spray ethanol on the body of the flasks everytime removing or keeping it in the incubator. I try not to spray the caps. I notice that my media gets cloudy in 24h as the cells lysed. A PHD student recommend me not to spray anymore, when I stop spraying, the condition get so much better, MCF7 becomes easily confluent. Why?

I posted my question as new topic just now, maybe you could give some of your opinion.

[isabellaweng]

bokashi farmer, on 04 September 2010 - 09:27 PM, said:

I dont know what is Incap cells, do you mind explaining?

I have similar problem with MCF-7. I start to notice that MCF-7 is might be sensitive to ethanol. I made it custom to spray ethanol on the body of the flasks everytime removing or keeping it in the incubator. I try not to spray the caps. I notice that my media gets cloudy in 24h as the cells lysed. A PHD student recommend me not to spray anymore, when I stop spraying, the condition get so much better, MCF7 becomes easily confluent. Why?

I posted my question as new topic just now, maybe you could give some of your opinion.

In some published paper, some people use 0.1%ethanol as vehicle control,but my 0.1%ethanol group were all dead, but 0.1%DMSO group was OK, maybe different resource, our MCF-7 cells were just sensitive to ethanol, now, I dissolved all my reagents in DMSO, my experiment is going on well. Good luck to you.