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In situ hybridization enigma


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#1 gabriella

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Posted 03 September 2010 - 03:06 AM

My in situ hybridization is a nightmare!!!!!
I perform it using a protocol that has worked very well for the first and second experiments. Then for next experiments the signal is very low and unspecific. I have not change the RNA probe and the tissue sample. Do anyone know which could be the weak link ?
Gabriella

#2 Rsm

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Posted 03 September 2010 - 07:23 AM

My in situ hybridization is a nightmare!!!!!
I perform it using a protocol that has worked very well for the first and second experiments. Then for next experiments the signal is very low and unspecific. I have not change the RNA probe and the tissue sample. Do anyone know which could be the weak link ?
Gabriella


Make a new probe. Check that your other reagents are RNAse-free, best way is to make all of them new.

Cheers,

rsm
I got soul, but I'm not a soldier




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