My in situ hybridization is a nightmare!!!!!
I perform it using a protocol that has worked very well for the first and second experiments. Then for next experiments the signal is very low and unspecific. I have not change the RNA probe and the tissue sample. Do anyone know which could be the weak link ?
Gabriella
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Rsm
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Posted 03 September 2010 - 07:23 AM
gabriella, on 03 September 2010 - 03:06 AM, said:
My in situ hybridization is a nightmare!!!!!
I perform it using a protocol that has worked very well for the first and second experiments. Then for next experiments the signal is very low and unspecific. I have not change the RNA probe and the tissue sample. Do anyone know which could be the weak link ?
Gabriella
I perform it using a protocol that has worked very well for the first and second experiments. Then for next experiments the signal is very low and unspecific. I have not change the RNA probe and the tissue sample. Do anyone know which could be the weak link ?
Gabriella
Make a new probe. Check that your other reagents are RNAse-free, best way is to make all of them new.
Cheers,
rsm
I got soul, but I'm not a soldier
