PVDF membrane dried after transfer (Western)
Posted 02 September 2010 - 06:19 PM
So after transferring, I cut the PVDF membrane so that it would fit into a case in which I could block with 5% milk. However, I guess I was too slow (it was my first time), so the membrane dried out and I was able to see the lanes. I just quickly put my membrane into the case and it is now blocking overnight at 4 degrees in 5% milk.
Do you think that everything is still going to be okay? How might this affect my results? Please help me! Thank you for your time.
Posted 02 September 2010 - 06:30 PM
Don't worry, I think it will be fine. Actually, I once went to a western blot seminar where a speaker recommended allowing the membrane to dry after transfer and before blocking because it allows for better protein detection. I don't know if this is true (I've never tried it myself), though I don't see why it would cause harm.
Best of luck with your first western!
Posted 02 September 2010 - 07:54 PM
I was told this at a seminar, too -- from a Millipore technical rep, I believe. I haven't tried it either...
Posted 02 September 2010 - 07:57 PM
"This is SPARTA!"
"I´m the goddamn batman"
Posted 02 September 2010 - 08:15 PM
Posted 02 September 2010 - 08:27 PM
genius does what it must
i do what i get paid to do
Posted 12 November 2010 - 04:17 AM