I'm sorry, but i 've never worked w/ phosphoinositides before... My goal is to reconstitute them and then spot different concentrations on a nitrocellulose membrane in order to perform lipid binding specificity assays.
I just got the package and realize they mention that they should not be reconstituted on plastic, nor handled w/ plastic tips...
any advice on how usually you perform these experiments?
Thank you so much!! any help is more then welcomed!!
Phosphoinisitides avanti - i'm supposed to reconstitute them in methanol:cho
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