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Protein quantification

Started by dt8511, Sep 02 2010 01:29 PM

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#1 dt8511

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Posted 02 September 2010 - 01:29 PM

I am trying to quantify a protein in cell lysate that is not well studied, so there are no good ELISA kits out there yet.  I have given up on troubleshooting the one ELISA I was able to find.  My PI does not want me using Western blot because it is not definitive enough for protein quantification. Any suggestions on the next best option for protein quantification?

#2 yufang7

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Posted 02 September 2010 - 06:06 PM

Radioimmunoassay

#3 rachelhauser

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Posted 05 March 2011 - 02:39 PM

Lowry method/Bradford method.
"Live Long and Prosper".
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