I've recently started using white 96 well plates (from VWR) for my qPCR reactions (not sure if they make a difference but I am too paranoid at this point than to want to switch). I am using a homebrew mastermix cocktail which is a clear liquid that is very difficult to visualize on the white background of the plate. I've noticed that a few companies sell blue colored mastermixes to aid in visualization and protect against pippeting errors (e.g. http://www.abgene.co....asp?prodID=372 and http://www.quantabio.com/).
The only company that I can find that offers the reagent as a stand alone add-in is http://www.tataa.com...uct-flyer.html. However, they do not seem to offer the product in the U.S.
Any other suggested solutions (e.g. making the dye myself or other products)?
adding dyes to qPCR mix for visualization in white plates
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