Help Stained after blocking and won't unstain!!!
1 reply to this topic
Posted 02 September 2010 - 11:49 AM
I made a dumb mistake. I accidently stained my membrane with Ponceau S after staining for an hour!!! My whole membrane turned red! And will not unstain. Any advice??!!
Posted 02 September 2010 - 04:05 PM
Unfortunately there is little you can do about this (presuming you mean that you blocked for 1 hour and then Ponceau), other than lots of washes - try in blocking solution - it has protein so it should remove some of the stain at least.