Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

problems with yeast contamination


  • Please log in to reply
1 reply to this topic

#1 sara.r

sara.r

    sara.r

  • Active Members
  • PipPipPipPipPip
  • 87 posts
1
Neutral

Posted 02 September 2010 - 03:18 AM

HI
During last month I have had serious problem with yeast contamination, I tried several ways like decontamination and changing incubator , changing serum and supplements source , using new cell vials from cell bank and filtering all my old medium and addition of amphotrecin b to medium. using amphotricin contamination reduced but not eleminated I am wondering if yeat spores can pass trough .22 micro filters or remain stable during autoclaving? I cant find out the contamination source, is there any suggestions?
regards

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,738 posts
400
Excellent

Posted 02 September 2010 - 04:01 PM

The spores will not survive autoclaving or pass through a .2 um filter. The contamination could be coming from skin or hair - yeasts are extremely common on the skin and hair, make sure you wear gloves, have your hair tied back, and wear a resularly washed lab coat when working with cells. Be very careful with sterile technique too.

Also, if you bake bread or brew beer/wine, you may be bringing the yeast in from that.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.