Posted 31 August 2010 - 05:23 PM
I have long RNA more than , I need to use it in EMSA assay but I need to fragment it to get small size RNA's , is restriction digestion in specific sites, can any body explain how to chose the sites?
Posted 02 September 2010 - 07:06 AM
Posted 02 September 2010 - 05:06 PM
Restriction digestion of RNA's? Its impossible. You have to amplify smaller DNA templates. Next you can use it for generation of shorter RNA constructs.
I am sorry I didn't mean RNA, I ment DNA plasmid, so if I have long plasmid for RNA can I digest the areas that I need with restriction enzyme, and then I can use T7 for transcription of this fragmnets?
Posted 03 September 2010 - 01:07 AM