I'm looking for a good standart protocol for extracting RNA from relatively small amounts of human cells (5,000 - 10,000). Maybe here's anybody who could give me a hint, please?
Edited by Rumpel, 31 August 2010 - 06:00 AM.
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#1
Rumpel
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Posted 31 August 2010 - 05:41 AM
Hi,
I'm looking for a good standart protocol for extracting RNA from relatively small amounts of human cells (5,000 - 10,000). Maybe here's anybody who could give me a hint, please?
I'm looking for a good standart protocol for extracting RNA from relatively small amounts of human cells (5,000 - 10,000). Maybe here's anybody who could give me a hint, please?
#2
Curtis
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Posted 31 August 2010 - 06:58 AM
Rumpel, on 31 August 2010 - 05:41 AM, said:
Hi,
I'm looking for a good standart protocol for extracting RNA from relatively small amounts of human cells (5,000 - 10,000). Maybe here's anybody who could give me a hint, please?
I'm looking for a good standart protocol for extracting RNA from relatively small amounts of human cells (5,000 - 10,000). Maybe here's anybody who could give me a hint, please?
Most people use Trizol method, there is a comprehensive protocol on wikipedia covering all steps. This is not really a low amount to get RNA from. 10^4 will give you enough RNA.
#3
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Posted 31 August 2010 - 07:11 AM
Ok, thank you very much:)
