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RNA extraction protocol for small amounts of cells


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#1 Rumpel

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Posted 31 August 2010 - 05:41 AM

Hi,

I'm looking for a good standart protocol for extracting RNA from relatively small amounts of human cells (5,000 - 10,000). Maybe here's anybody who could give me a hint, please?

Edited by Rumpel, 31 August 2010 - 06:00 AM.


#2 Curtis

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Posted 31 August 2010 - 06:58 AM

Hi,

I'm looking for a good standart protocol for extracting RNA from relatively small amounts of human cells (5,000 - 10,000). Maybe here's anybody who could give me a hint, please?


Most people use Trizol method, there is a comprehensive protocol on wikipedia covering all steps. This is not really a low amount to get RNA from. 10^4 will give you enough RNA.

#3 Rumpel

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Posted 31 August 2010 - 07:11 AM

Ok, thank you very much:)




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