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PCR with one primer


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#1 Ameya P

Ameya P

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Posted 30 August 2010 - 09:46 PM

Hello All,

I came across this paper published in 1992, where the author has used only the forward primer for his PCR. He has mentioned a range for PCR product with each of the primers used.

My question is, if I have to repeat this experiment, how can I exercise control over the size of PCR product and make it consistent? or is it that the length of the product will be dependent on extension times alone???

Thanks
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#2 gebirgsziege

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Posted 30 August 2010 - 10:53 PM

as a rule of thumb 1 min elongation at 72C is needed for a product of 1000 bp length, but not sure if this is of any help for your experiment.
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