PCR with one primer
Posted 30 August 2010 - 09:46 PM
I came across this paper published in 1992, where the author has used only the forward primer for his PCR. He has mentioned a range for PCR product with each of the primers used.
My question is, if I have to repeat this experiment, how can I exercise control over the size of PCR product and make it consistent? or is it that the length of the product will be dependent on extension times alone???
NEW!!!! FISH on Wheels on CoffeeTableScience!!!!
Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist
Posted 30 August 2010 - 10:53 PM