PCR with one primer
Posted 30 August 2010 - 09:46 PM
I came across this paper published in 1992, where the author has used only the forward primer for his PCR. He has mentioned a range for PCR product with each of the primers used.
My question is, if I have to repeat this experiment, how can I exercise control over the size of PCR product and make it consistent? or is it that the length of the product will be dependent on extension times alone???
NEW!!!! A Raft like none other! on CoffeeTableScience!!!!
Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist
Posted 30 August 2010 - 10:53 PM