PCR with one primer
Posted 30 August 2010 - 09:46 PM
I came across this paper published in 1992, where the author has used only the forward primer for his PCR. He has mentioned a range for PCR product with each of the primers used.
My question is, if I have to repeat this experiment, how can I exercise control over the size of PCR product and make it consistent? or is it that the length of the product will be dependent on extension times alone???
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Posted 30 August 2010 - 10:53 PM