Posted 30 August 2010 - 05:20 AM
Is there anyone who is working with radioactive materials. I have to do the assay for transporters in oocytes of Xenopus. Every paper tells the concentration as 1mci/ ml. What I am not able to get is the amount in ml that has to be added for the assays. If anyone can help me please reply. Desperate to start the experiment, but not able to get the standardize the protocol. For example for 14 C Adenosine the conc is 98.6ug/ml as given in the product data sheet. I dont know how much Iam supposed to add from the stock and how to dilute it. Any help will be greatly appreciated.
Posted 30 August 2010 - 05:35 PM
Posted 01 September 2010 - 07:48 AM
Posted 01 September 2010 - 10:57 AM