CD/CD8 ratio in Lymphnodes
Posted 30 August 2010 - 05:19 AM
I found a paper were they wrote here the Paper that they found only CD4+ Tcells in the LN of immunized mice no CD8+. Isn't that kid of strange shouldd't be there at least some CD8 cells.
How would be in general the Ratio between the populations change by immunization?
Posted 30 August 2010 - 06:57 PM
Edited by leelee, 30 August 2010 - 06:58 PM.
Posted 30 August 2010 - 10:06 PM
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Posted 31 August 2010 - 12:07 AM
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Edited by leelee, 31 August 2010 - 12:08 AM.
Posted 31 August 2010 - 12:15 AM
Edited by leelee, 31 August 2010 - 12:16 AM.
Posted 31 August 2010 - 12:44 AM
If you see the FACS-picture (Fig.2) there they wrote: "after staining with FITC-conjugated mAb to mouse CD4 or CD8."
But when you have a look at the picture you see almost no other cells then CD4 cells. So there are 96,8% CD4 cells? The rest are other cells, but obviously not all other cells could be CD8 positiv cells. the normal CD4/CD8 Ratio should be around 1:3 to 1:4 what I know.
So do you have such change from CD8 to CD4 seen before in mice that get immunized?
Thank you for the quick reply.
Posted 31 August 2010 - 09:07 PM
"Flow-cytometry analysis of peptide-activated LN cells showed that both Coch 131–150 (Figure (Figure2A)2A) and β-tectorin 71–90 (Figure (Figure2B)2B) preferentially activated CD4+ T cells."
Additionally, whilst they mention CD8 T cells in the figure description, the actually figure itself has fluorescence intensity on the X axis as FITC/CD4 so they haven't actually shown the CD8 stained data at all.
Edited by leelee, 31 August 2010 - 09:08 PM.