my protein is cysteine rich and i am getting it in insoluble fraction. i have tried growing bacteria under low temperature conditions but the protein is still present in insoluble fraction. what should i do to bring them in soluble fraction???????????
to bring protein in soluble fraction
1 reply to this topic
Posted 30 August 2010 - 06:18 AM
Hola, You don´t say us what E. coli strain use for expression, but for improving cytoplasmic disulphide bridges any of Origami or Rosetta-gamy 2 are recommended because they are trxB and gor 522. if I were you I would choose any of Origami B except lacI, and i would try growing it at 25-30 ēC analyzing expression without and light induction 0->0.1 mM IPTG. Good luck