Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

to bring protein in soluble fraction


  • Please log in to reply
1 reply to this topic

#1 sdar

sdar

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 30 August 2010 - 01:58 AM

my protein is cysteine rich and i am getting it in insoluble fraction. i have tried growing bacteria under low temperature conditions but the protein is still present in insoluble fraction. what should i do to bring them in soluble fraction???????????

#2 protolder

protolder

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 293 posts
9
Neutral

Posted 30 August 2010 - 06:18 AM

Hola, You dont say us what E. coli strain use for expression, but for improving cytoplasmic disulphide bridges any of Origami or Rosetta-gamy 2 are recommended because they are trxB and gor 522. if I were you I would choose any of Origami B except lacI, and i would try growing it at 25-30 C analyzing expression without and light induction 0->0.1 mM IPTG. Good luck




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.