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Problem with gel extraction of DNA.


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#1 RAP

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Posted 28 August 2010 - 09:40 PM

I was doing gel extraction of my DNA today and after cutting the band portion, I mistakenly dropped it on floor. I know it is foolish and carelessness. Anyways, I still proceeded to extract the DNA. In the mini column which I was using for clean up, I found little dust particles. Do you think that there might be some contamination in my DNA?

#2 pito

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Posted 29 August 2010 - 08:09 AM

It could be.. but since you allready did the extraction and proceeded.. you can continue and see what happens. If it wont work.. well too bad, if it does work... :D

I once had a similar problem: dropped a part of some DNA bands on the table, just used it as the others and didnt see any difference in the end..
I however did an extra cleaning step (I do it always): ethanonpurification.
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.




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