I was trying to PCR-amplify using a primer of around 20bp and it work.
Now when I check my data it seems to me that the primer they gave me to work with has a difference of 7bp in relation to the template DNA.
I wanted to ask if 7bp difference between primer and DNA template should normally make the PCR not work. In this case I might have entered the data (the primer sequence) in my notebook incorrecly.
Or would the PCR still work with the 7bp difference?
Edited by humalog, 28 August 2010 - 01:27 PM.